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KMID : 0624620130460020113
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BMB Reports
2013 Volume.46 No. 2 p.113 ~ p.118
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Overexpression of TTRAP inhibits cell growth and induces apoptosis in osteosarcoma cells
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Zhou Caihong
Shen Qi
Xue Jinglun
Ji Chaoneng
Chen Jinzhong
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Abstract
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TTRAP is a multi-functional protein that is involved in multiple aspects of cellular functions including cell proliferation, apoptosis and the repair of DNA damage. Here, we demonstrated that the lentivirus-mediated overexpression of TTRAP significantly inhibited cell growth and inducedapoptosis in osteosarcoma cells. The ectopic TTRAP suppressed the growth and colony formation capacity of two osteosarcoma cell lines, U2OS and Saos-2. Cell apoptosis was induced in U2OS cells and the cell cycle was arrested at G2/M phase in Saos-2 cells. Exogenous expression of TTRAP in serum-starved U2OS and Saos-2 cells induced an increase in caspase-3/-7 activity and a decrease in cyclin B1 expression. In comparison with wild-typeTTRAP, mutations in the 5'-tyrosyl-DNA phosphodiesterase activity of TTRAP, in particularTTRAP(E152A), showed decreased inhibitory activity on cell growth. These results may aid in clarifying the physiological functions of TTRAP, especially its roles in the regulation of cell growthand tumorigenesis.
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KEYWORD
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Apoptosis, Growth inhibition, Osteosarcoma, TTRAP, Tyrosyl DNA phosphodiesterase
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